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Adsorption of Soluble Immunoglobulin‐Type Adhesion Molecules to Cellulose Acetate Beads
Author(s) -
Nishise Shoichi,
Takeda Yuji,
Nara Hidetoshi,
Abe Yasuhiko,
Sasaki Yu,
Asao Hironobu,
Ueno Yoshiyuki
Publication year - 2018
Publication title -
therapeutic apheresis and dialysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.415
H-Index - 53
eISSN - 1744-9987
pISSN - 1744-9979
DOI - 10.1111/1744-9987.12693
Subject(s) - incubation , medicine , cellulose acetate , adsorption , monocyte , granulocyte , adhesion , cell adhesion molecule , in vitro , intercellular adhesion molecule 1 , incubation period , chromatography , cellulose , immunology , biochemistry , chemistry , organic chemistry
Circulating levels of soluble intercellular adhesion molecule‐1 (sICAM‐1) and vascular adhesion molecule‐1 (sVCAM‐1) are elevated in patients with inflammatory bowel disease. Cellulose acetate (CA) beads are used as carriers for granulocyte and monocyte (GM) adsorptive apheresis (GMA). We investigated the effect of CA beads on sICAM‐1 and sVCAM‐1 plasma concentrations in vitro. Because GM adsorption to CA beads increased with a rise in the incubation temperature in our previous study, peripheral blood was incubated with and without CA beads at 5, 25, 37, or 43 °C and plasma sICAM‐1 and sVCAM‐1 was measured. The sICAM‐1 and sVCAM‐1 concentrations in samples incubated with CA beads were significantly lower than those without CA beads at all four temperatures. However, no significant differences were observed both sICAM‐1 and sVCAM‐1 plasma levels at the four different temperatures after incubation with CA beads. These results suggest that independent of incubation temperature, sICAM‐1 and sVCAM‐1 are likely to adsorb CA beads. These molecules may be a new index for predicting the therapeutic effects of GMA.