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Removal Characteristics of Immunoadsorption With the Immusorba TR‐350 Column Using Conventional and Selective Plasma Separators
Author(s) -
Ohkubo Atsushi,
Okado Tomokazu,
Miyamoto Satoko,
Goto Keigo,
Yamamoto Motoki,
Maeda Takuma,
Itagaki Ayako,
Seshima Hiroshi,
Kurashima Naoki,
Sohara Eisei,
Uchida Shinichi,
Rai Tatemitsu
Publication year - 2016
Publication title -
therapeutic apheresis and dialysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.415
H-Index - 53
eISSN - 1744-9987
pISSN - 1744-9979
DOI - 10.1111/1744-9987.12467
Subject(s) - medicine , immunoadsorption , chromatography , column (typography) , immunology , antibody , mechanical engineering , chemistry , connection (principal bundle) , engineering
In Japan, immunoadsorption (IA) is performed using a conventional plasma separator and Immusorba TR‐350 column (TR‐350) for the treatment of neurological immune diseases. By this method, TR‐350 has the limited maximal capacity of the immunoglobulin G (IgG) adsorption, and fibrinogen (Fbg) is reduced remarkably. Evacure EC‐4A10 (EC‐4A) is a selective plasma separator and the sieving coefficients of IgG and Fbg using EC‐4A were 0.5 and 0, respectively. Here, we investigated the removal characteristics of IgG and Fbg in IA by TR‐350 using two different plasma membrane separators: conventional plasma separator (PE‐IA) and EC‐4A (EC‐IA). In vitro filtration using plasma effluent was performed with a closed circuit. When the processed volume was 3 L, estimated removal amounts by PE‐IA were 3172 mg for IgG and 3329 mg for Fbg, respectively. When the processed volume was 3 L, estimated removal amounts by EC‐IA were 4946 mg and 1916 mg, respectively. EC‐IA can be considered useful for the removal of IgG, including auto‐antibodies, while retaining Fbg, thereby allowing even daily use.