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Flexible manipulation of Omb levels in the endogenous expression region of Drosophila wing by combinational overexpression and suppression strategy
Author(s) -
Zhang XuBo,
Dong Wei,
Li KaiXia,
Wang JuanJuan,
Shen Jie,
Moussian Bernard,
Zhang JianZhen
Publication year - 2020
Publication title -
insect science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 45
eISSN - 1744-7917
pISSN - 1672-9609
DOI - 10.1111/1744-7917.12705
Subject(s) - rna interference , biology , transgene , psychological repression , drosophila melanogaster , endogeny , gene expression , gene , microbiology and biotechnology , regulation of gene expression , genetics , wing , gene knockdown , rna , biochemistry , engineering , aerospace engineering
Manipulating an exogenous or endogenous gene of interest at a defined level is critical for a wide variety of experiments. The Gal4/UAS system has been widely used to direct gene expression for studying complex genetic and biological problems in Drosophila melanogaster and other model organisms. Driven by a given tissue‐specific Gal4, expressing UAS‐transgene or UAS‐RNAi (RNA interference) could be used to up‐ or down‐regulate target gene expression, respectively. However, the efficiency of the Gal4/UAS system is roughly predefined by properties of transposon vector constructs and the insertion site in the transgenic stock. Here, we describe a simple way to modulate optomotor blind ( omb ) expression levels in its endogenous expression region of the wing disc. We co‐expressed UAS‐omb and UAS‐omb‐RNAi together under the control of dpp‐Gal4 driver which is expressed in the omb expression region of the wing pouch. The repression effect is more sensitive to temperature than that of overexpression. At low temperature, overexpression plays a dominant role but the efficiency is attenuated by UAS‐omb‐RNAi. In contrast, at high temperature RNAi predominates in gene expression regulation. By this strategy, we could manipulate omb expression levels at a moderate level. It allows us to manipulate omb expression levels in the same tissue between overexpression and repression at different stages by temperature control.