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High throughput sequencing reveals Drosophila suzukii responses to insecticides
Author(s) -
Mishra Ruchir,
Chiu Joanna C.,
Hua Gang,
Tawari Nilesh R.,
Adang Michael J.,
Sial Ashfaq A.
Publication year - 2018
Publication title -
insect science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 45
eISSN - 1744-7917
pISSN - 1672-9609
DOI - 10.1111/1744-7917.12498
Subject(s) - biology , drosophila suzukii , drosophila (subgenus) , throughput , dna sequencing , genetics , drosophila melanogaster , drosophilidae , gene , engineering , telecommunications , wireless
Global climate change and acquired resistance to insecticides are threats to world food security. Drosophila suzukii , a devastating invasive pest in many parts of the world, causes substantial economic losses to fruit production industries, forcing farmers to apply broad‐spectrum insecticides frequently. This could lead to the development of insecticide resistance. We determined the Lethal Concentration 50 (median lethal concentration, LC 50 ) values of zeta‐cypermethrin, spinosad, and malathion insecticides against D. suzukii colonies established from Clarke and Pierce county Georgia, United States. The LC 50 values were 3 fold higher in the Pierce county population for all insecticide treatments. We then used RNA sequencing to analyze the responses of Pierce and Clarke population flies surviving a LC 50 treatment of the 3 insecticides. We identified a high number of differentially expressed genes that are likely involved in detoxification and reduced cuticular penetration, especially in the Pierce population, with extensive overlap in differentially expressed genes between the 3 insecticide treatments. Finally, we predicted fewer nonsynonymous single nucleotide variants having deleterious effects on protein function among detoxification, insecticide target, and cuticular protein encoding genes in Pierce flies. Thus a combination of increased gene expression and fewer deleterious single nucleotide variants highlights molecular mechanisms underlying the higher LC 50 values for Pierce population flies.