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Localization of Banana bunchy top virus and cellular compartments in gut and salivary gland tissues of the aphid vector Pentalonia nigronervosa
Author(s) -
Watanabe Shizu,
Borthakur Dulal,
Bressan Alberto
Publication year - 2016
Publication title -
insect science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 45
eISSN - 1744-7917
pISSN - 1672-9609
DOI - 10.1111/1744-7917.12211
Subject(s) - biology , endosome , microbiology and biotechnology , endocytosis , clathrin , internalization , salivary gland , midgut , caveolae , cellular compartment , actin , golgi apparatus , cell , endoplasmic reticulum , signal transduction , botany , biochemistry , larva , intracellular
Banana bunchy top virus (BBTV) (Nanoviridae: Babuvirus) is transmitted by aphids of the genus Pentalonia in a circulative manner. The cellular mechanisms by which BBTV translocates from the anterior midgut to the salivary gland epithelial tissues are not understood. Here, we used multiple fluorescent markers to study the distribution and the cellular localization of early and late endosomes, macropinosomes, lysosomes, microtubules, actin filaments, and lipid raft subdomains in the gut and principal salivary glands of Pentalonia nigronervosa . We applied colabeling assays, to colocalize BBTV viral particles with these cellular compartments and structures. Our results suggest that multiple potential cellular processes, including clathrin‐ and caveolae‐mediated endocytosis and lipid rafts, may not be involved in BBTV internalization.