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Hedgehog signaling pathway regulated the target genes for adipogenesis in silkworm Bombyx mori
Author(s) -
Liang Shuang,
Chen RuiTing,
Zhang DengPan,
Xin HuHu,
Lu Yan,
Wang MeiXian,
Miao YunGen
Publication year - 2015
Publication title -
insect science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 45
eISSN - 1744-7917
pISSN - 1672-9609
DOI - 10.1111/1744-7917.12164
Subject(s) - bombyx mori , adipogenesis , biology , gene , hedgehog signaling pathway , hedgehog , bombyx , microbiology and biotechnology , crispr , genetics
Hedgehog (Hh) signals regulate invertebrate and vertebrate development, yet the role of the pathway in adipose development remains poorly understood. In this report, we found that Hh pathway components are expressed in the fat body of silkworm larvae. Functional analysis of these components in a Bm N cell line model revealed that activation of the Hh gene stimulated transcription of Hh pathway components, but inhibited the expression of the adipose marker gene AP2. Conversely, specific RNA interference‐mediated knockdown of Hh resulted in increased AP2 expression. This further showed the regulation of Hh signal on the adipose marker gene. In silkworm larval models, enhanced adipocyte differentiation and an increase in adipocyte cell size were observed in silkworms that had been treated with a specific Hh signaling pathway antagonist, cyclopamine. The fat‐body‐specific Hh blockade tests were consistent with Hh signaling inhibiting silkworm adipogenesis. Our results indicate that the role of Hh signaling in inhibiting fat formation is conserved in vertebrates and invertebrates.