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Expression analysis of chlorophyllid α binding protein, a secretory, red fluorescence protein in the midgut of silkworm, Bombyx mori
Author(s) -
Chen Lin,
Yang Rui,
Hu XiaoLong,
Xiang XingWei,
Wu XiaoFeng
Publication year - 2014
Publication title -
insect science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 45
eISSN - 1744-7917
pISSN - 1672-9609
DOI - 10.1111/1744-7917.12026
Subject(s) - midgut , bombyx mori , biology , instar , messenger rna , microbiology and biotechnology , larva , biochemistry , gene , botany
Chlorophyllid α binding protein ( chbp ) was recently characterized by its ability to bind the prosthetic group of chlorophylls and little information is known regarding its expression. In the present study, we found that chpb was expressed highly and exclusively in the midgut of silkworm, Bombyx mori . The expression level of chbp was very high in the newly molted fifth instar larvae followed by gradual decline in the same instar. Our results demonstrated that CHBP was a secretory protein and located mainly in the apical of midgut epithelial cells. Real‐time polymerase chain reaction analysis results showed that chpb highly expressed in the anterior midgut, threefold and sixfold higher compared with that of the middle midgut and posterior midgut, respectively, and chpb expression declined in darkness. In addition, the expression of chbp was affected by high‐dose virus or bacterium infection.