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The Stability of 4‐Chloromethcathinone in Blood and Vitreous Humor
Author(s) -
Nowak Karolina,
Szpot Paweł,
Zawadzki Marcin
Publication year - 2020
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/1556-4029.14454
Subject(s) - chromatography , mass spectrometry , chemistry , biological materials , medicine , biomedical engineering
We present results of our study on the stability of 4‐chloromethcathinone (4‐CMC) in authentic postmortem peripheral blood and vitreous humor samples. The stability of 4‐CMC was determined in postmortem blood samples (for a period of 90 days) and vitreous humor (30 days) at three different temperatures: −15°C, +4°C, and + 23°C. The analyses were carried out using ultra‐high‐performance liquid chromatography coupled with triple‐quadrupole tandem mass spectrometry (UHPLC‐QqQ‐MS/MS). In both materials, the lowest 4‐CMC stability was demonstrated at room temperature. The blood samples stored in a freezer (−15°C) showed stability for the entire study period (90 days), while in the case of the vitreous humor sample stored at the same temperature the concentration of the substance decreased by 53% after 30 days. The study carried out in authentic postmortem blood and vitreous humor samples confirms the previous reports of 4‐CMC instability in biological material. Authors suggest that the biological material should be stored frozen until analyses are carried out as soon as possible after collection of the material.