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Results of the 2018 Rapid DNA Maturity Assessment
Author(s) -
Romsos Erica L.,
French Julie L.,
Smith Mark,
Figarelli Vincent,
Harran Frederick,
Vandegrift Glenn,
Moreno Lilliana I.,
Callaghan Thomas F.,
Brocato Joanie,
Vaidyanathan Janaki,
Pedroso Juan C.,
Amy Andrea,
Stoiloff Stephanie,
Morillo Victor H.,
Czetyrko Karina,
Johnson Elizabeth D.,
Tagyos Jessica,
Murray Ashley,
Vallone Peter M.
Publication year - 2020
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/1556-4029.14267
Subject(s) - genotyping , dna profiling , typing , genetics , dna , genotype , biology , computational biology , gene
Three commercially available integrated rapid DNA instruments were tested as a part of a rapid DNA maturity assessment in July of 2018. The assessment was conducted with sets of blinded single‐source reference samples provided to participants for testing on the individual rapid platforms within their laboratories. The data were returned to the National Institute of Standards and Technology (NIST) for review and analysis. Both FBI‐defined automated review (Rapid DNA Analysis) and manual review (Modified Rapid DNA Analysis) of the datasets were conducted to assess the success of genotyping the 20 Combined DNA Index System (CODIS) core STR loci and full profiles generated by the instruments. Genotype results from the multiple platforms, participating laboratories, and STR typing chemistries were combined into a single analysis. The Rapid DNA Analysis resulted in a success rate of 80% for full profiles (85% for the 20 CODIS core loci) with automated analysis. Modified Rapid DNA Analysis resulted in a success rate of 90% for both the CODIS 20 core loci and full profiles (all attempted loci per chemistry). An analysis of the peak height ratios demonstrated that 95% of all heterozygous alleles were above 59% heterozygote balance. For base‐pair sizing precision, the precision was below the standard 0.5 bp deviation for both the ANDE 6C System and the RapidHIT 200.