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Α Simple Method for the Determination of Lacosamide in Blood by GC ‐ MS
Author(s) -
Mouskeftara Thomai,
Alexandridou Anastasia,
Krokos Adamantios,
Gika Helen,
Mastrogianni Orthodoxia,
Orfanidis Amvrosios,
Raikos Nikolaos
Publication year - 2020
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/1556-4029.14151
Subject(s) - lacosamide , chromatography , extraction (chemistry) , chemistry , blood concentration , therapeutic drug monitoring , epilepsy , pharmacology , medicine , drug , psychiatry
Lacosamide is a functionalized amino acid with antiepileptic function. Therapeutic drug monitoring ( TDM ) in patients for lacosamide is critical as it allows clinicians to control epileptic seizures. A single liquid–liquid extraction step was applied for the extraction of lacosamide from whole blood samples which were thereafter analyzed by GC ‐ MS . Optimum extraction conditions were selected on the basis of experiments with various solvents at different pH s, indicating ethyl acetate at pH 12 as the most efficient parameters for the extraction of lacosamide. Method exhibited linearity from 2 to 100 μg/ mL with R 2 = 0.998. Accuracy and precision were evaluated at three concentrations and found to be within acceptable limits. LOD and LOQ were determined at 0.1 and 0.5 μg/ mL , respectively. Lacosamide was found to be stable at storage conditions. The developed method was applied successfully in clinical samples and postmortem blood sample from an overdose case.