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Combined DNA Typing and Protein Identification from Unfired Brass Cartridges , , ,
Author(s) -
Sterling StaceyAnn,
Mason Katelyn E.,
Anex Deon S.,
Parker Glendon J.,
Hart Bradley,
Prinz Mechthild
Publication year - 2019
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/1556-4029.14042
Subject(s) - cartridge , typing , brass , dna , dna profiling , genomic dna , chromatography , chemistry , biology , materials science , biochemistry , genetics , metallurgy , organic chemistry , copper
Biological evidence analysis from contact traces is adversely affected by low quantity and quality of DNA . Proteins in these samples contain potentially individualizing information and may be particularly important for difficult surfaces such as brass, where DNA may yield incomplete profiles. In this study, touched unfired brass cartridges were sampled using dry tape or wet swabs and analyzed by separating DNA and protein from the same collected material, thus producing both genomic and proteomic information. DNA recovery was similar for both collection methods, with tape yielding an average of 1.36 ± 1.87 ng and swabs, 1.34 ± 3.04 ng. Analysis by mass spectrometry identified 95 proteins, with the two collection methods showing no significant difference ( p = 0.76) in the average number of collected proteins: 44.5 ± 10.9, (tape) versus 47.9 ± 20.4 (swabs). Proteins can be collected from fingerprints at levels necessary to provide identifying information, thus expanding information obtained from challenging evidence.