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Evaluation of DNA Extraction Methods from Waterlogged Bones: A Pilot Study ,
Author(s) -
Cartozzo Claire,
Singh Baneshwar,
Boone Edward,
Simmons Tal
Publication year - 2018
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/1556-4029.13792
Subject(s) - extraction (chemistry) , dna extraction , rib cage , dna , genomic dna , chromatography , biology , biomedical engineering , chemistry , anatomy , medicine , polymerase chain reaction , biochemistry , gene
When deaths occur in water, soft tissue decomposes after a temperature‐dependent period, making DNA identification dependent on bone. This study examined the effects of water on bone DNA purity and quantity, and determined the best of three extraction methods for isolating DNA . The organic phenol–chloroform method consistently extracted DNA approximating the accepted 260/280 purity value (~1.8); ChargeSwitch® gDNA Plant Kit and DN easy Blood and Tissue Kit produced fair and unacceptable values, respectively. The purity value for humerus and rib samples was consistent across accumulated degree days ( ADD ). Significant differences in quantification among extraction methods and between bone types were identified. Ribs and ChargeSwitch® gDNA Plant Kit samples produced the lowest mean Ct values of the bone types and the extraction methods, respectively. Therefore, this study proposes that magnetic bead technology extraction methods and ribs be considered when processing bones that have been submerged in water for any length of time.