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Massively Parallel Sequencing of Forensic STR s Using the Ion Chef™ and the Ion S5™ XL Systems ,
Author(s) -
Wang Le,
Chen Man,
Wu Bo,
Liu YiCheng,
Zhang GuangFeng,
Jiang Li,
Xu XiuLan,
Zhao XingChun,
Ji AnQuan,
Ye Jian
Publication year - 2018
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/1556-4029.13767
Subject(s) - massive parallel sequencing , genotyping , ion semiconductor sequencing , microsatellite , dna profiling , str analysis , dna sequencing , forensic science , genotype , computational biology , genetics , allele , biology , dna , gene
Next‐generation sequencing ( NGS ) has been used to genotype forensic short tandem repeat ( STR ) markers for individual identification and kinship analysis. STR data from several NGS platforms have been published, but forensic application trials using the Ion S5™ XL system have not been reported. In this work, we report sensitivity, reproducibility, mixture, simulated degradation, and casework sample data on the Ion Chef™ and S5™ XL systems using an early access 25‐plex panel. Sensitivity experiments showed that over 97% of the alleles were detectable with down to 62 pg input of genomic DNA . In mixture studies, alleles from minor contributors were correctly assigned at 1:9 and 9:1 ratios. NGS successfully gave 12 full genotype results from 13 challenging casework samples, compared with five full results using the CE platform. In conclusion, the Ion Chef™ and the Ion S5™ XL systems provided an alternative and promising approach for forensic STR genotyping.