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Evaluation Of A Powder‐Free DNA Extraction Method For Skeletal Remains
Author(s) -
Harrel Michelle,
Mayes Carrie,
Gangitano David,
HughesStamm Sheree
Publication year - 2018
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/1556-4029.13749
Subject(s) - dna extraction , extraction (chemistry) , dna , chromatography , ex vivo , microsatellite , grinding , polymerase chain reaction , chemistry , microbiology and biotechnology , materials science , biology , biochemistry , gene , composite material , allele , in vitro
Abstract Bones are often recovered in forensic investigations, including missing persons and mass disasters. While traditional DNA extraction methods rely on grinding bone into powder prior to DNA purification, the TB one Ex buffer ( DNA Chip Research Inc.) digests bone chips without powdering. In this study, six bones were extracted using the TB one Ex kit in conjunction with the PrepFiler ® BTA ™ DNA extraction kit (Thermo Fisher Scientific) both manually and via an automated platform. Comparable amounts of DNA were recovered from a 50 mg bone chip using the TB one Ex kit and 50 mg of powdered bone with the PrepFiler ® BTA ™ kit. However, automated DNA purification decreased DNA yield ( p < 0.05). Nevertheless, short tandem repeat ( STR ) success was comparable across all methods tested. This study demonstrates that digestion of whole bone fragments is an efficient alternative to powdering bones for DNA extraction without compromising downstream STR profile quality.