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STR Genotyping from a Dry‐Cleaned Skirt in a Sexual Assault Case
Author(s) -
Ogawa Hisae,
Hiroshige Yuuji,
Yoshimoto Takashi,
Ishii Akira,
Yamamoto Toshimichi
Publication year - 2018
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/1556-4029.13698
Subject(s) - sexual assault , genotyping , microsatellite , stain , semen , crime scene , str analysis , blood stains , typing , dna profiling , forensic examination , sperm , biology , multiplex , staining , genetics , dna , poison control , pathology , medicine , genotype , criminology , psychology , forensic engineering , injury prevention , allele , environmental health , engineering , gene
In this sexual assault case, the standard preliminary semen examinations could not confirm physically or biochemically whether the accused's semen had stained the victim's skirt because the skirt had been dry‐cleaned for stain removal and had been worn for more than a year after the assault. Fortunately, however, a photograph taken just after the assault was found in the court records that showed white stains on the checkered skirt. The locations of the stains were estimated based on the checkered pattern of the fabric, and microscopic examination using Baecchi's staining revealed the presence of spermatozoa. Further analysis indicated the male DNA profile generated from the sperm cells was consistent with the suspect's DNA using three multiplex STR typing systems for a total of 21 autosomal and 17 Y chromosomal short tandem repeats ( STR s). Ultimately, the result of the DNA profile played a very useful role as additional evidence.