Premium
Evaluation of Commercial‐off‐the‐Shelf Materials for the Preservation of Bacillus anthracis Vegetative Cells for Forensic Analysis ,
Author(s) -
Angelini Daniel J.,
Harris Jacquelyn V.,
Burton Laura L.,
Rastogi Pooja R.,
Smith Lisa S.,
Rastogi Vipin K.
Publication year - 2018
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/1556-4029.13549
Subject(s) - bacillus anthracis , spore , contamination , biology , microbiology and biotechnology , bacteria , ecology , genetics
Environmental surface sampling is crucial in determining the zones of contamination and overall threat assessment. Viability retention of sampled material is central to such assessments. A systematic study was completed to determine viability of vegetative cells under nonpermissive storage conditions. Despite major gains in nucleic acid sequencing technologies, initial positive identification of threats must be made through direct culture of the sampled material using classical microbiological methods. Solutions have been developed to preserve the viability of pathogens contained within clinical samples, but many have not been examined for their ability to preserve biological agents. The purpose of this study was to systematically examine existing preservation materials that can retain the viability of Bacillus anthracis vegetative cells stored under nonpermissive temperatures. The results show effectiveness of five of seventeen solutions, which are capable of retaining viability of a sporulation deficient strain of B. anthracis Sterne when stored under nonrefrigerated conditions.