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Evaluation of Skin Surface as an Alternative Source of Reference DNA Samples: A Pilot Study
Author(s) -
Albujja Mohammed H.,
Bin Dukhyil Abdul Aziz,
Chaudhary Abdul Rauf,
Kassab Ahmed Ch.,
Refaat Ahmed M.,
Babu Saranya Ramesh,
Okla Mohammad K.,
Kumar Sachil
Publication year - 2018
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/1556-4029.13468
Subject(s) - buccal swab , dna , dna extraction , buccal administration , cotton swab , genomic dna , chromatography , biology , microbiology and biotechnology , medicine , chemistry , polymerase chain reaction , dentistry , genetics , gene
An acceptable area for collecting DNA reference sample is a part of the forensic DNA analysis development. The aim of this study was to evaluate skin surface cells ( SSC ) as an alternate source of reference DNA sample. From each volunteer ( n = 10), six samples from skin surface areas (forearm and fingertips) and two traditional samples (blood and buccal cells) were collected. Genomic DNA was extracted and quantified then genotyped using standard techniques. The highest DNA concentration of SSC samples was collected using the tape/forearm method of collection (2.1 ng/μL). Cotton swabs moistened with ethanol yielded higher quantities of DNA than swabs moistened with salicylic acid, and it gave the highest percentage of full STR profiles (97%). This study supports the use of SSC as a noninvasive sampling technique and as a extremely useful source of DNA reference samples among certain cultures where the use of buccal swabs can be considered socially unacceptable.