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Changes in Attenuated Total Reflection Fourier Transform Infrared Spectra as Blood Dries Out
Author(s) -
Zhang Yinming,
Wang Qi,
Li Bing,
Wang Zhijun,
Li Chengzhi,
Yao Yao,
Huang Ping,
Wang Zhenyuan
Publication year - 2017
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/1556-4029.13324
Subject(s) - attenuated total reflection , absorbance , fourier transform infrared spectroscopy , analytical chemistry (journal) , human blood , fourier transform , infrared spectroscopy , spectroscopy , reflection (computer programming) , infrared , chemistry , materials science , optics , chromatography , mathematics , medicine , physiology , physics , computer science , organic chemistry , programming language , mathematical analysis , quantum mechanics
The time since deposition ( TSD ) of a bloodstain is a valuable piece of evidence for forensic scientists to determine the time at which a crime took place. The objective of this study was to determine whether attenuated total reflection ( ATR ) Fourier transform infrared ( FTIR ) spectroscopy could be used to estimate the TSD of a bloodstain in a relatively early period (from 0 min to the time required for the bloodstain to dry out). For this purpose, we used ATR ‐ FTIR to study the variation in absorbance at certain wavelengths as rat and human blood sample dried out. The absorbance at 3308/cm (A3308) was found to have a close correlation with the TSD during this time period, and the changes in A3308 during the drying of rat and human blood drops under the same controlled conditions showed similar results. The current study indicates that ATR ‐ FTIR spectroscopy has potential as a tool for estimating TSD at early time periods of blood deposition.