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Effectiveness of Coupled Application of AmpFℓSTR Yfiler Kit and Reduced Size Y‐chromosomal Short Tandem Repeat Analysis for Archeological Human Bones
Author(s) -
Oh Chang Seok,
Lee Soong Deok,
Shin KyoungJin,
Shin Dong Hoon
Publication year - 2016
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/1556-4029.12950
Subject(s) - y str , biology , tandem , computational biology , genetics , microsatellite , gene , engineering , allele , aerospace engineering
The A mpFℓ STR Y filer PCR A mplification ( Y filer) kit continues to be improved for a better analytical efficiency in cases of highly degraded DNA . The authors endeavored to determine whether coupling of the Y filer kit with supplemental multiplex amplification of some Y ‐ STR loci is a more efficient analytical mode for poorly preserved human femurs ( n = 15) discovered at K orean archeological sites. To reveal locus profiles not easily obtained by Y filer analysis, custom‐designed primers were adopted for the DYS 390, DYS 391, DYS 392, DYS 438, DYS 439, and DYS 635 loci. The success rate for 16 Y ‐ STR locus profiles obtained from the 15 femurs was improved from 18.33% (in the use of Y filer kit only) to 49.17% (the coupled use of Y filer and custom‐designed primers). In this study, the authors established that the custom‐designed primers offer a markedly improved success rate for obtainment of Y ‐ STR profiles from degraded aDNA not easily identified by sole use of the Y filer assay.