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The Influence of Selected Fingerprint Enhancement Techniques on Forensic DNA Typing of Epithelial Cells Deposited on Porous Surfaces
Author(s) -
Tsai LiChin,
Lee ChengChang,
Chen ChunChieh,
Lee James ChunI,
Wang ShengMeng,
Huang NuEn,
Linacre Adrian,
Hsieh HsingMei
Publication year - 2016
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/1556-4029.12893
Subject(s) - fingerprint (computing) , dna , dna profiling , acetic acid , chemistry , chromatography , porosity , typing , silver nitrate , biology , biochemistry , genetics , nuclear chemistry , organic chemistry , computer science , artificial intelligence
Fingerprints deposited at crime scene can be a source of DNA . Previous reports on the effects of fingerprint enhancement methods have focused mainly on fingermarks deposited in blood or saliva. Here, we evaluate the effects of fingerprint enhancement methods on fingerprints deposited on porous surfaces. We performed real‐time quantification and STR typing, the results of which indicated that two methods (iodine fuming and 1,2‐indanedione in ethyl acetate enhancement) had no effect on the quantity of DNA isolated and resultant STR alleles when compared to control samples. DNA quantities and allele numbers were lower for samples enhanced with silver nitrate and 1,2‐indanedione in acetic acid when compared to control samples. Based on DNA quantity, quality, and observable stochastic effects, our data indicated that iodine fuming and 1,2‐indanedione in ethyl acetate were the preferred options for the enhancement of fingerprints on porous surfaces.