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Near Infrared Spectroscopy Detection and Quantification of Herbal Medicines Adulterated with Sibutramine
Author(s) -
Silva Neirivaldo Cavalcante,
Honorato Ricardo Saldanha,
Pimentel Maria Fernanda,
Garrigues Salvador,
Cervera Maria Luisa,
Guardia Miguel
Publication year - 2015
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/1556-4029.12884
Subject(s) - sibutramine , herbal supplement , near infrared spectroscopy , traditional medicine , calibration , multivariate statistics , medicine , partial least squares regression , mathematics , statistics , weight loss , alternative medicine , pathology , obesity , physics , quantum mechanics
There is an increasing demand for herbal medicines in weight loss treatment. Some synthetic chemicals, such as sibutramine ( SB ), have been detected as adulterants in herbal formulations. In this study, two strategies using near infrared ( NIR ) spectroscopy have been developed to evaluate potential adulteration of herbal medicines with SB : a qualitative screening approach and a quantitative methodology based on multivariate calibration. Samples were composed by products commercialized as herbal medicines, as well as by laboratory adulterated samples. Spectra were obtained in the range of 14,000–4000 per cm. Using PLS ‐ DA , a correct classification of 100% was achieved for the external validation set. In the quantitative approach, the root mean squares error of prediction ( RMSEP ), for both PLS and MLR models, was 0.2%w/w. The results prove the potential of NIR spectroscopy and multivariate calibration in quantifying sibutramine in adulterated herbal medicines samples.