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A Direct Aqueous Derivatization GSMS Method for Determining Benzoylecgonine Concentrations in Human Urine
Author(s) -
Chericoni Silvio,
Stefanelli Fabio,
Da Valle Ylenia,
Giusiani Mario
Publication year - 2015
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/1556-4029.12880
Subject(s) - benzoylecgonine , chromatography , derivatization , chemistry , urine , extraction (chemistry) , chloroformate , detection limit , gas chromatography–mass spectrometry , calibration curve , quantitative analysis (chemistry) , mass spectrometry , solid phase extraction , biochemistry
A sensitive and reliable method for extraction and quantification of benzoylecgonine ( BZE ) and cocaine ( COC ) in urine is presented. Propyl‐chloroformate was used as derivatizing agent, and it was directly added to the urine sample: the propyl derivative and COC were then recovered by liquid–liquid extraction procedure. Gas chromatography–mass spectrometry was used to detect the analytes in selected ion monitoring mode. The method proved to be precise for BZE and COC both in term of intraday and interday analysis, with a coefficient of variation ( CV ) <6%. Limits of detection ( LOD ) were 2.7 ng/mL for BZE and 1.4 ng/mL for COC . The calibration curve showed a linear relationship for BZE and COC ( r 2 >0.999 and >0.997, respectively) within the range investigated. The method, applied to thirty authentic samples, showed to be very simple, fast, and reliable, so it can be easily applied in routine analysis for the quantification of BZE and COC in urine samples.