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Evaluation of Direct PCR Amplification Using Various Swabs and Washing Reagents
Author(s) -
Altshuler Hallie,
Roy Reena
Publication year - 2015
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/1556-4029.12865
Subject(s) - microbiology and biotechnology , virology , chromatography , chemistry , biology
Abstract DNA profiles were generated via direct amplification from blood and saliva samples deposited on various types of swab substrates. Each of the six non‐ FTA substrates used in this research was punched with a Harris 1.2 mm puncher. After 0.1 μL of blood or 0.5 μL saliva, samples were deposited on each of these punches, samples were pretreated with one of four buffers and washing reagents. Amplification was performed using direct and nondirect autosomal and Y‐ STR kits. Autosomal and Y‐ STR profiles were successfully generated from most of these substrates when pretreated with buffer or washing reagents. Concordant profiles were obtained within and between the six substrates, the six amplification kits, and all four reagents. The direct amplification of substrates which do not contain lysing agent would be beneficial to the forensic community as the procedure can be used on evidence samples commonly found at crime scenes.