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Comparison of Four Saliva Detection Methods to Identify Expectorated Blood Spatter
Author(s) -
Park HeeYeon,
Son BuNam,
Seo YoungIl,
Lim SiKeun
Publication year - 2015
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/1556-4029.12864
Subject(s) - saliva , chromatography , medicine , chemistry
Blood spatter analysis is an important step for crime scene reconstruction. The presence of saliva in blood spatter could indicate expectorated blood which is difficult to distinguish from impact spatter. In this study, four saliva test methods ( SALI g AE ® , Phadebas ® sheet, RSID ™ ‐Saliva kit, and starch gel diffusion) were compared to identify the best method for detecting expectorated blood spatter. The RSID ™ ‐Saliva kit showed the highest sensitivity even when saliva was mixed with blood, and was not inhibited by the presence of blood. The SALI g AE ® test provided easy and rapid results, but the yellow color of a positive reaction was overwhelmed by the red color of the blood. The starch gel diffusion method and the Phadebas ® sheet exhibited relatively low sensitivity and the assay took a long time. When using the RSID ™ ‐Saliva kit for identifying saliva in blood, results should be read within 10 min.