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Fatty Acid Profiles for Differentiating Growth Medium Formulations Used to Culture Bacillus cereus T‐strain Spores
Author(s) -
Ehrhardt Christopher J.,
Murphy Devonie L.,
Robertson James M.,
Bannan Jason D.
Publication year - 2015
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/1556-4029.12771
Subject(s) - spore , bacillus cereus , food science , strain (injury) , cereus , biology , fatty acid , casein , nutrient , fructose , growth medium , microbiology and biotechnology , biochemistry , chemistry , bacteria , ecology , genetics , anatomy
Microbial biomarkers that indicate aspects of an organism's growth conditions are important targets of forensic research. In this study, we examined fatty acid composition as a signature for the types of complex nutrients in the culturing medium. Bacillus cereus T‐strain spores were grown in medium formulations supplemented with one of the following: peptone (meat protein), tryptone (casein protein), soy protein, and brain–heart infusion. Cellular biomass was profiled with fatty acid methyl ester ( FAME ) analysis. Results showed peptone cultures produced spores enriched in straight‐chained lipids. Tryptone cultures produced spores enriched in branched‐odd lipids when compared with peptone, soy, and brain–heart formulations. The observed FAME variation was used to construct a set of discriminant functions that could help identify the nutrients in a culturing recipe for an unknown spore sample. Blinded classification tests were most successful for spores grown on media containing peptone and tryptone, showing 88% and 100% correct identification, respectively.