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Identification of Saliva Using Micro RNA Biomarkers for Forensic Purpose
Author(s) -
Wang Zheng,
Zhang Ji,
Wei Wei,
Zhou Di,
Luo Haibo,
Chen Xiaogang,
Hou Yiping
Publication year - 2015
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/1556-4029.12730
Subject(s) - saliva , rna , body fluid , taqman , computational biology , microrna , biology , real time polymerase chain reaction , microbiology and biotechnology , medicine , genetics , gene , pathology , biochemistry
In the forensic science community, micro RNA (mi RNA ) profiling has started to be explored as an alternative tool for body fluid identification. Several origins of body fluid can be distinguished by measuring differential expression patterns of particular mi RNA s. However, most of reported saliva mi RNA s are nonoverlapping and debatable. The aim of this study was to develop a strategy of identifying saliva using mi RNA biomarkers for forensic purpose. Eight mi RNA candidates were selected to examine expression abundance in forensically relevant body fluids using hydrolysis probes quantitative real‐time PCR (TaqMan qPCR ). Results revealed that none of them was truly saliva specific, and only miR‐200c‐3p, miR‐203a, and miR‐205‐5p were higher or more moderate expression in saliva. A stepwise strategy that combines each of three mi RNA s with different body fluid‐specific mi RNA s was developed, and three mi RNA combinations could effectively differentiate saliva from other body fluids.