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Mucosal Cell Isolation and Analysis from Cellular Mixtures of Three Contributors
Author(s) -
Xu Cheng,
Feng Lei,
Yang Fan,
Jia Jing,
Ji AnQuan,
Hu Lan,
Li CaiXia
Publication year - 2015
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/1556-4029.12715
Subject(s) - dna , saliva , somatic cell , isolation (microbiology) , genotype , microbiology and biotechnology , biology , dna profiling , chromatography , chemistry , computational biology , genetics , bioinformatics , gene , biochemistry
In forensic genetic analyses, mixtures of various biological materials are common samples. Micromanipulation, which is performed based on differences in cellular morphology, is an effective method for the isolation of cells from mixtures. In this study, mucosal cell was isolated from somatic cellular mixtures (blood and saliva) based on micromanipulation and a low volume‐ PCR ( LV ‐ PCR ) platform. One hundred and twenty‐six parallel LV ‐ PCR processes were performed using an Identifiler ® kit, with 107 reactions yielding single‐source DNA profiles. Among them, 54 full profiles (50%) and 37 partial profiles (13–15 loci) were obtained. Based on the above method, we obtained a single‐source DNA profile from a cigarette butt contaminated by two victims’ blood in a murder case. The generated genotype was used to query a DNA database, and a perfect match was found.