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Effectiveness of Single‐Nucleotide Polymorphisms to Investigate Cattle Rustling
Author(s) -
Fernández María E.,
RogbergMuñoz Andrés,
Lirón Juan P.,
Goszczynski Daniel E.,
Ripoli María V.,
Carino Mónica H.,
PeralGarcía Pilar,
Giovambattista Guillermo
Publication year - 2014
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/1556-4029.12562
Subject(s) - snp , single nucleotide polymorphism , forensic science , biology , genetics , repeatability , microsatellite , dna profiling , snp array , genotype , dna , statistics , gene , mathematics , allele
Short tandem repeats ( STR )s have been the eligible markers for forensic animal genetics, despite single‐nucleotide polymorphisms ( SNP )s became acceptable. The technology, the type, and amount of markers could limit the investigation in degraded forensic samples. The performance of a 32‐ SNP panel genotyped through OpenArrays TM (real‐time PCR based) was evaluated to resolve cattle‐specific forensic cases. DNA from different biological sources was used, including samples from an alleged instance of cattle rustling. SNP s and STR s performance and repeatability were compared. SNP call rate was variable among sample type (average = 80.18%), while forensic samples showed the lowest value (70.94%). The repeatability obtained (98.7%) supports the used technology. SNP s had better call rates than STR s in 12 of 20 casework samples, while forensic index values were similar for both panels. In conclusion, the 32‐ SNP s used are as informative as the standard bovine STR battery and hence are suitable to resolve cattle rustling investigations.