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A Time Course Study Demonstrating m RNA , micro RNA , 18 S r RNA , and U 6 sn RNA Changes to Estimate PMI in Deceased Rat's Spleen
Author(s) -
Lv Yehui,
Ma Kaijun,
Zhang Heng,
He Meng,
Zhang Ping,
Shen Yiwen,
Jiang Nan,
Ma Duan,
Chen Long
Publication year - 2014
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/1556-4029.12447
Subject(s) - rna , messenger rna , microbiology and biotechnology , chemistry , biology , biochemistry , gene
Determining the postmortem interval ( PMI ) is important in criminal, civil, and forensic cases. We examined the feasibility of using the transcript abundances of m RNA s, 18 S r RNA , U 6 sn RNA , and micro RNA s as a means to estimate the PMI . We removed spleen tissues from rats at different PMI s under 4°C or 25°C and examined gene transcript abundances in these samples by RT ‐q PCR . Using the algorithm geNorm, we found that micro RNA s to be appropriate control markers because they were less affected by PMI and temperature. We also characterized relationships between observed PMI and the transcript levels of the above‐mentioned RNA s. GAPDH 1 and ACTB 1 fluctuated slightly like cubic curves, while GAPDH 2 and ACTB 2 decreased rapidly. 18 S r RNA transcript level exhibited a parabolic‐like trend at 25°C and exponential growth at 4°C, while U 6 transcript level exhibited exponential decay at 25°C and a parabolic‐like trend at 4°C. Following validation, we conclude that GAPDH 2, ACTB 2, and 18 S r RNA are suitable makers in the accurate determination of PMI .