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Constructing STR Multiplexes for Individual Identification of Hungarian Red Deer
Author(s) -
Szabolcsi Zoltan,
Egyed Balazs,
Zenke Petra,
Padar Zsolt,
Borsy Adrienn,
Steger Viktor,
Pasztor Erzsebet,
Csanyi Sandor,
Buzas Zsuzsanna,
Orosz Laszlo
Publication year - 2014
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/1556-4029.12403
Subject(s) - microsatellite , identification (biology) , biology , allele , genetics , evolutionary biology , similarity (geometry) , zoology , statistics , mathematics , ecology , artificial intelligence , computer science , gene , image (mathematics)
Red deer is the most valuable game of the fauna in Hungary, and there is a strong need for genetic identification of individuals. For this purpose, 10 tetranucleotide STR markers were developed and amplified in two 5‐plex systems. The study presented here includes the flanking region sequence analysis and the allele nomenclature of the 10 loci as well as the PCR optimization of the DeerPlex I and II . LD pairwise tests and cross‐species similarity analyses showed the 10 loci to be independently inherited. Considerable levels of genetic differences between two subpopulations were recorded, and F ST was 0.034 using AMOVA . The average probability of identity ( PI ave ) was at the value of 2.6736 × 10 −15 . This low value for PI ave nearly eliminates false identification. An illegal hunting case solved by DeerPlex is described herein. The calculated likelihood ratio ( LR ) illustrates the potential of the 10 red deer microsatellite markers for forensic investigations.