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Analysis of Positive Control STR Experiments Reveals that Results Obtained for FGA , D3S1358, and D13S317 Condition the Success Rate of the Analysis of Routine Reference Samples
Author(s) -
Murigneux Valentine,
Dufour AnneBéatrice,
Lobry Jean R.,
Pène Laurent
Publication year - 2014
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/1556-4029.12360
Subject(s) - locus (genetics) , spectrum analyzer , sensitivity (control systems) , multivariate analysis , statistics , exploratory analysis , multivariate statistics , analytical chemistry (journal) , biology , genetics , microbiology and biotechnology , mathematics , chromatography , chemistry , computer science , engineering , gene , data science , electronic engineering , telecommunications
About 120,000 reference samples are analyzed each year in the F orensic L aboratory of L yon. A total of 1640 positive control experiments used to validate and optimize the analytical method in the routine process were submitted to a multivariate exploratory data analysis approach with the aim of better understanding the underlying sources of variability. The peak heights of the 16 genetic markers targeted by the AmpFℓ STR ® Identifiler ® STR kit were used as variables of interest. Six different 3130xl genetic analyzers located in the same controlled environment were involved. Two major sources of variability were found: (i) the DNA load of the sample modulates all peak heights in a similar way so that the 16 markers are highly correlated, (ii) the genetic analyzer used with a locus‐specific response for peak height and a better sensitivity for the most recently acquired. Three markers ( FGA , D3S1358, and D13S317) were found to be of special interest to predict the success rate observed in the routine process.