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D esigner P sychostimulants in U rine by L iquid C hromatography– T andem M ass S pectrometry ,
Author(s) -
Kerrigan Sarah,
Mott Ashley,
Jatzlau Breanna,
Ortiz Francisco,
Perrella Laura,
Martin Sarah,
Bryand Kelsie
Publication year - 2014
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/1556-4029.12306
Subject(s) - chemistry , detection limit , chromatography , matrix (chemical analysis)
Designer psychostimulants are known by recreational drug users to produce a complex array of adrenergic and hallucinogenic effects. Many of these drugs are not targeted during routine toxicology testing and as a consequence, they are rarely reported. The purpose of this study was to develop a procedure for the detection of 15 psychostimulants in urine using liquid chromatography–tandem mass spectrometry ( LC ‐ MS / MS ), specifically 2,5‐dimethoxy‐4‐bromophenethylamine (2 C ‐ B ), 2,5‐dimethoxy‐4‐chlorophenethylamine (2 C ‐ C ), 2,5‐dimethoxy‐4‐methylphenethylamine (2 C ‐ D ), 2,5‐dimethoxy‐4‐ethylphenethylamine (2C‐ E ), 2,5‐dimethoxyphenethylamine (2 C ‐ H ), 2,5‐dimethoxy‐4‐iodophenethylamine (2 C ‐ I ), 2,5‐dimethoxy‐4‐ethylthiophenethylamine (2 C ‐ T ‐2), 2,5‐dimethoxy‐4‐isopropylthiophenethylamine (2 C ‐ T ‐4), 2,5‐dimethoxy‐4‐propylthiophenethylamine (2 C ‐ T ‐7), 2,5‐dimethoxy‐4‐bromoamphetamine ( DOB ), 2,5‐dimethoxy‐4‐chloroamphetamine ( DOC ), 2,5‐dimethoxy‐4‐ethylamphetamine ( DOET ), 2,5‐dimethoxy‐4‐iodoamphetamine ( DOI ), 2,5‐dimethoxy‐4‐methylamphetamine ( DOM ), and 4‐methylthioamphetamine (4‐ MTA ). Analytical recoveries using solid‐phase extraction were 64–92% and the limit of detection was 0.5 ng/mL for all drugs except 2 C ‐ B (1 ng/mL). The assay was evaluated in terms of analytical recovery, precision, accuracy, linearity, matrix effect, and interferences. The technique allows for the simultaneous detection of 15 psychostimulants at sub‐ng/ mL concentrations.