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Preliminary Study on Diffuse Axonal Injury by Fourier Transform Infrared Spectroscopy Histopathology Imaging
Author(s) -
Yang Tiantong,
He Guanglong,
Zhang Xiang,
Chang Lin,
Zhang Haidong,
Ripple Mary G.,
Fowler David R.,
Li Ling
Publication year - 2014
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/1556-4029.12290
Subject(s) - fourier transform infrared spectroscopy , white matter , diffuse axonal injury , histopathology , amide , chemistry , pathology , traumatic brain injury , immunohistochemistry , medicine , biochemistry , magnetic resonance imaging , optics , radiology , physics , psychiatry
The objective of this study was to evaluate the application of F ourier transform infrared ( FTIR ) spectroscopy for detecting diffuse axonal injury ( DAI ) in a mouse model. Brain tissues from DAI mouse model were prepared with H&E, silver, and β‐amyloid precursor protein (β– APP ) immunohistochemistry stains and were also studied with FTIR . The infrared spectrum images showed high absorption of amide II in the subcortical white matter of the experimental mouse brain, while there was no obvious expression of amide II in the control mouse brain. The areas with high absorption of amide II were in the same distribution as the DAI region confirmed by the silver and β‐ APP studies. The result suggests that high absorption of amide II correlates with axonal injury. The use of FTIR imaging allows the biochemical changes associated with DAI pathologies to be detected in the tissues, thus providing an important adjunct method to the current conventional pathological diagnostic techniques.