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A 21‐Locus Autosomal SNP Multiplex and its Application in Forensic Science
Author(s) -
Hou Guangwei,
Jiang Xianhua,
Yang Yanyan,
Jia Fei,
Li Qiang,
Zhao Jinling,
Guo Fei,
Liu Limin
Publication year - 2014
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/1556-4029.12259
Subject(s) - multiplex , locus (genetics) , amplicon , single nucleotide polymorphism , genotyping , genetics , snp genotyping , typing , snp , biology , forensic identification , str multiplex system , molecular inversion probe , multiplex polymerase chain reaction , population , amelogenin , microbiology and biotechnology , computational biology , genotype , polymerase chain reaction , gene , medicine , environmental health
Abstract To develop a cost‐effective technique for single‐nucleotide polymorphism (SNP) genotyping and improve the efficiency to analyze degraded DNA, we have established a novel multiplex system including 21‐locus autosomal SNPs and amelogenin locus, which was based on allele‐specific amplification (ASA) and universal reporter primers (URP). The target amplicons for each of the 21 SNPs arranged from 63 base pair (bp) to 192 bp. The system was tested in 539 samples from three ethnic groups (Han, Mongolian, and Zhuang population) in China, and the total power of discrimination (TPD) and cumulative probability of exclusion (CPE) were more than 0. and 0.98, respectively. The system was further validated with forensic samples and full profiles could be achieved from degraded DNA and 63 case‐type samples. In summary, the multiplex system offers an effective technique for individual identification of forensic samples and is much more efficient in the analysis of degraded DNA compared with standard STR typing.