Premium
A Comparison of DNA Extraction Using A uto M ate E xpress™ and EZ 1 Advanced XL from Liquid Blood, Bloodstains, and Semen Stains
Author(s) -
Fujii Koji,
Inokuchi Shota,
Kitayama Tetsushi,
Nakahara Hiroaki,
Mizuno Natsuko,
Sekiguchi Kazumasa
Publication year - 2013
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/1556-4029.12174
Subject(s) - semen , blood stains , dna extraction , lysis , lysis buffer , biology , microsatellite , dna , microbiology and biotechnology , polymerase chain reaction , chromatography , andrology , chemistry , genetics , medicine , gene , allele
In this study, DNA was extracted using an AutoMate Express™ and an EZ 1 Advanced XL from liquid blood, fresh and aged bloodstains, and fresh and aged semen stains. Extracted DNA was quantified by real‐time PCR using the D17Z1 locus. Short tandem repeat typing was performed using an AmpFℓSTR ® Identifiler kit. The yields of DNA obtained by the AutoMate Express™ were higher from fresh bloodstains and fresh semen stains, almost the same from aged bloodstains and aged semen stains, but slightly lower from liquid blood compared with those obtained by the EZ 1 Advanced XL . The addition of dithiothreitol or the use of PrepFiler™ lysis buffer improved the EZ 1 Advanced XL results from fresh bloodstains, but not for liquid blood and aged bloodstains. Our results demonstrated that the P rep F iler™ lysis buffer is the main contributor to the higher DNA yields of the A uto M ate Express™ for fresh bloodstains.