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Association of the eukaryotic vaginal virome with prophylactic antibiotic exposure and reproductive outcomes in a subfertile population undergoing in vitro fertilisation: a prospective exploratory study
Author(s) -
Eskew AM,
Stout MJ,
Bedrick BS,
Riley JK,
Omurtag KR,
Jimenez PT,
Odem RR,
Ratts VS,
Keller SL,
Jungheim ES,
Wylie KM
Publication year - 2020
Publication title -
bjog: an international journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.157
H-Index - 164
eISSN - 1471-0528
pISSN - 1470-0328
DOI - 10.1111/1471-0528.15951
Subject(s) - human virome , population , early pregnancy loss , azithromycin , gynecology , pregnancy , obstetrics , in vitro fertilisation , medicine , embryo transfer , biology , antibiotics , gestation , genetics , environmental health , gene , metagenomics
Objective The objective of this study was to use high‐throughput sequencing to describe the vaginal eukaryotic DNA virome in patients undergoing in vitro fertilisation (IVF) to examine associations between the vaginal virome, antibiotic exposure and IVF outcomes. Design Prospective exploratory study. Setting Single academic fertility centre. Population Subfertile women age 18–43 years undergoing their first IVF cycle with a fresh embryo transfer. Methods The primary exposure was prophylactic azithromycin or no azithromycin before IVF. A mid‐vaginal swab was obtained at the time of embryo transfer for virome analysis. Main outcome measures The primary outcomes compared between exposure groups were characteristics of vaginal virome and clinical pregnancy rates. Secondary outcomes were virome associations with number of oocytes retrieved, number of blastocysts and implantation rate. Results Twenty‐six women contributed a vaginal swab before embryo transfer. There were no significant differences in IVF outcomes between azithromycin groups. There was no association between viral diversity and clinical pregnancy overall. A higher diversity of herpesviruses and α‐papillomaviruses was observed in samples from the azithromycin‐treated group compared with the no azithromycin group ( P = 0.04). In women that received azithromycin, viral diversity was higher in the group that did not achieve clinical pregnancy compared with those who did ( P = 0.06). Conclusions We demonstrate that the vaginal eukaryotic virome in women undergoing IVF is associated with antibiotic exposure. Additionally, we demonstrate an inverse trend between viral diversity and pregnancy, with a higher number of viruses detected associated with failure to achieve clinical pregnancy in the azithromycin group. Tweetable abstract Higher viral diversity is associated with prophylactic antibiotic exposure in subfertile women undergoing IVF.