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FAS A‐670G and Fas ligand IVS2nt A 124G polymorphisms are significantly increased in women with pre‐eclampsia and may contribute to HELLP syndrome: a case‐controlled study
Author(s) -
Raguema N,
Zitouni H,
Ben Ali Gannoun M,
Benletaifa D,
Almawi WY,
Mahjoub T,
Lavoie JL
Publication year - 2018
Publication title -
bjog: an international journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.157
H-Index - 164
eISSN - 1471-0528
pISSN - 1470-0328
DOI - 10.1111/1471-0528.15412
Subject(s) - fas ligand , hellp syndrome , eclampsia , medicine , haemolysis , genotyping , preeclampsia , immunology , exact test , gastroenterology , endocrinology , genotype , apoptosis , pregnancy , gene , biology , genetics , programmed cell death
Objective We evaluated the association between the Fas‐670A/G and the Fas ligand FasL IVS2nt 124 A/G polymorphisms and the risk of pre‐eclampsia and its complications. Design A case‐controlled study. Setting University Hospitals in most areas of Tunisia. Population We recruited 300 pregnant women who developed pre‐eclampsia and 300 age‐matched healthy pregnant women from the same hospital. Methods Genotyping of Fas‐670A/G and the FasL IVS2nt 124A/G gene polymorphisms were conducted using polymerase chain reaction‐restriction fragment length polymorphism among our cohort. Main outcome measures Fisher's exact test was used to compare the statistical differences between groups for categorical variables and Student t tests were used for continuous variables. Results The frequency of the Fas‐670G gene variant was significantly increased in women with pre‐eclampsia (42%) compared with control women (30%; P < 0.001). Also, a statistically significant difference was obtained in the distribution of the FasL IVS2nt 124G gene variant when comparing women with pre‐eclampsia (43%) with controls (30%; P < 0.001). Interestingly, we found that the carriage of Fas‐670G was associated with increased liver enzymes, suggesting an increased prevalence of the haemolysis, elevated liver enzymes and low platelets (HELLP) syndrome, a pre‐eclampsia complication. Conclusion The Fas‐670G and FasL IVS2nt 124G polymorphisms are associated with a higher risk of pre‐eclampsia and its complications. Tweetable abstract Polymorphisms in the Fas and FasL genes are associated with increased risk of pre‐eclampsia and HELLP syndrome.