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Circulating micro RNA expression profiles in pre‐eclampsia: evidence of increased miR‐885‐5p levels
Author(s) -
Sandrim VC,
Luizon MR,
Palei AC,
TanusSantos JE,
Cavalli RC
Publication year - 2016
Publication title -
bjog: an international journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.157
H-Index - 164
eISSN - 1471-0528
pISSN - 1470-0328
DOI - 10.1111/1471-0528.13903
Subject(s) - eclampsia , microrna , microvesicles , fold change , extracellular vesicle , andrology , real time polymerase chain reaction , extracellular , reverse transcription polymerase chain reaction , biology , reverse transcriptase , polymerase chain reaction , gene expression , medicine , gene , microbiology and biotechnology , pregnancy , genetics
Objective To validate and to compare the circulating micro RNA (miR) expression profiles between pre‐eclampsia and healthy pregnant women, to perform correlation analysis of the differently expressed miRs with clinical and biochemical parameters, and to verify the extracellular localisation of miRs in apoptotic bodies, microvesicles, and exosomes. Design A case–control study with a replication study. Setting Pregnant women attending maternity hospitals in Southeastern Brazil. Population Two obstetric white populations: a case–control study (19 pre‐eclampsia and 14 healthy pregnant) and a replication study (eight pre‐eclampsia and eight healthy pregnant). Methods PCR ‐array with 84 different miRs was performed in plasma from five pre‐eclampsia and four healthy pregnant women. In the case–control study, differently expressed miRs were validated using quantitative real‐time reverse transcription polymerase chain reaction ( qRT ‐ PCR ), and correlated with clinical and biochemical parameters. The plasma was then fractioned to study the extracellular localisation of miRs. Main outcome measures Gene expression profiles of miRs. Results From PCR ‐array, three miRs (miR‐376c‐3p, miR‐19a‐3p, and miR‐19b‐3p) were found to be down‐regulated and the miR‐885‐5p was found to be up‐regulated in pre‐eclampsia compared with healthy pregnant women. In the validation step, miR‐885‐5p was the only significantly different miR (fold‐change = 5.0, P < 0.05), which was confirmed in the replication study (fold‐change = 4.5, P < 0.05). Moreover, miR‐885‐5p was significantly correlated with the hepatic enzyme aspartate transaminase ( r = 0.66; P = 0.0034) and it was mostly associated with the exosomes (32‐fold higher than apoptotic bodies). Conclusions miR‐885‐5p is increased in plasma from pre‐eclampsia compared with healthy pregnant women, and it is released into circulation mainly inside exosomes. Tweetable Abstract miR‐885‐5p is increased in pre‐eclampsia and is released into circulation mainly inside exosomes.