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Circular RNA in blood corpuscles combined with plasma protein factor for early prediction of pre‐eclampsia
Author(s) -
Zhang YG,
Yang HL,
Long Y,
Li WL
Publication year - 2016
Publication title -
bjog: an international journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.157
H-Index - 164
eISSN - 1471-0528
pISSN - 1470-0328
DOI - 10.1111/1471-0528.13897
Subject(s) - circular rna , confidence interval , eclampsia , gestation , medicine , pregnancy , obstetrics , gestational age , microarray , preeclampsia , rna , andrology , gastroenterology , biology , gene expression , gene , genetics
Objective To determine the expression of circular RNA (circ RNA ) in blood corpuscles of pregnant women before 20 weeks of pregnancy and to create a new model to identify the performance of circ RNA combined with protein factors for the early diagnosis of pre‐eclampsia ( PE ). Design Nested case–control study. Setting University medical centre, Guangzhou, China. Population A total of 1400 pregnant women recruited between 8 and 20 weeks of gestation. In all, 41 women with PE were included in the study, and were matched with 41 normally pregnant women based on maternal age and gestational age at same‐size ratio. Methods The samples were analysed using a human circ RNA microarray in the discovery phase, then the circ RNA and the plasma protein factor endoglin ( ENG ) were validated. Finally we combined ENG with circ RNA to create a new early prediction model for PE . Main outcome measures Early changes of circ RNA and ENG in PE . Results The circ_101222 levels in blood corpuscles of patients with PE were significantly higher than those in corresponding healthy women ( P < 0.001). Using ENG in combination with circ_101222 resulted in a sensitivity of 0.7073, a specificity of 0.8049, and overall area under the curve of 0.876 (95% confidence interval 0.816–0.922) for the prediction of PE . Conclusion Circ RNA and plasma proteins may have some predictive value for PE (such as circ_101222 and ENG ). The performance of each of these factors may be strengthened when plasma proteins are used in combination with circ RNA . The results are preliminary and need to be validated in larger studies and other populations. Tweetable abstract Plasma protein endoglin in combination with circ_101222 strengthened the predictive power for pre‐eclampsia.