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Comparative appraisal of HPLC, Chloramine‐T and Lane–Eynon methods for quantification of carbohydrates in concentrated dairy products
Author(s) -
da Silva Luana Cristina A,
Lafetá Junior José A Q,
Leite Maurício O,
Fontes Edimar A F,
Coimbra Jane S R
Publication year - 2020
Publication title -
international journal of dairy technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.061
H-Index - 53
eISSN - 1471-0307
pISSN - 1364-727X
DOI - 10.1111/1471-0307.12710
Subject(s) - lactose , high performance liquid chromatography , chloramine t , chromatography , chemistry , sucrose , carbohydrate , food science , biochemistry , organic chemistry
Lactose and sucrose were quantified in sweetened condensed milk and ‘Dulce de leche’ using a high‐performance liquid chromatography (HPLC) approach as compared with the classical methods (Chloramine‐T and Lane–Eynon). Lane–Eynon method estimated the lowest values of carbohydrates for both products. The chromatographic method quantified the carbohydrate contents at the same range as Chloramine‐T method. The values of the estimated standard error (ESE) for HPLC versus Chloramine‐T (0.55–0.77 mass %) were lower than the ESE for HPLC versus Lane–Eynon (1.01–1.63 mass %). Calcium concentration in the products may explain underestimated carbohydrate contents by the Lane–Eynon method.