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Selection and analysis of bacteriophage‐insensitive mutants of Streptococcus thermophilus isolated in Ukraine
Author(s) -
Naumenko Oksana,
Skrypkina Inessa,
Zhukova Yaroslava,
Vakulenko Mykola,
Kigel Natalia
Publication year - 2019
Publication title -
international journal of dairy technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.061
H-Index - 53
eISSN - 1471-0307
pISSN - 1364-727X
DOI - 10.1111/1471-0307.12607
Subject(s) - streptococcus thermophilus , bacteriophage , mutant , virulence , biology , microbiology and biotechnology , fermentation , selection (genetic algorithm) , food science , genetics , gene , escherichia coli , computer science , lactobacillus , artificial intelligence
Bacteriophage‐insensitive Streptococcus thermophilus mutants ( BIM s) were obtained by treating the phage‐sensitive industrial strains with virulent phages. Five BIM s acquired resistance to S. thermophilus phages of two ( cos and pac ) main groups selected at Ukrainian dairy processing enterprises. In addition, the valuable biotechnological properties of BIM s were characterised. During fermentation, they formed fermented milk curds with a homogeneous, dense consistency, with pleasant taste and flavour. The BIM s were identified as S. thermophilus by phenotypic and species‐specific PCR methods. The BIM s could be used as starters to stabilise the fermentation process under phage infection conditions.