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A novel method for elimination of aflatoxin M1 in milk using Lactobacillus rhamnosus GG biofilm
Author(s) -
Assaf Jean Claude,
Khoury André El,
Chokr Ali,
Louka Nicolas,
Atoui Ali
Publication year - 2019
Publication title -
international journal of dairy technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.061
H-Index - 53
eISSN - 1471-0307
pISSN - 1364-727X
DOI - 10.1111/1471-0307.12578
Subject(s) - lactobacillus rhamnosus , biofilm , aflatoxin , food science , chemistry , atomic force microscopy , contamination , microbiology and biotechnology , lactobacillus , bacteria , biology , materials science , nanotechnology , ecology , fermentation , genetics
This study aimed to develop a new method for detoxification of milk from aflatoxin M1 ( AFM 1) by using Lactobacillus rhamnosus GG biofilm. After inoculation of milk contaminated with AFM 1 into L. rhamnosus GG biofilm, the unbound AFM 1 was extracted and quantified by HPLC . The stability of the formed AFM 1/biofilm complex using different AFM 1 contamination levels of milk was also studied. We found that the percentages of bound AFM 1 by L. rhamnosus GG biofilm reached up to 60.74%. While no significant difference in milk proteins content was observed after AFM 1 binding, some changes in total dry matter and fat content were noticed.