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Optimisation of Bacillus cereus biofilm removal in the dairy industry using an in vitro model of cleaning‐in‐place incorporating serine protease
Author(s) -
Kumari Sarita,
Sarkar Prabir K
Publication year - 2018
Publication title -
international journal of dairy technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.061
H-Index - 53
eISSN - 1471-0307
pISSN - 1364-727X
DOI - 10.1111/1471-0307.12454
Subject(s) - biofilm , bacillus cereus , protease , serine protease , chemistry , microbiology and biotechnology , cereus , response surface methodology , food science , matrix (chemical analysis) , pulp and paper industry , biology , bacteria , chromatography , biochemistry , enzyme , engineering , genetics
This article addresses a major hygienic issue in the dairy industry, namely biofilm removal. Response surface methodology was deployed to optimise Bacillus cereus biofilm removal conditions using serine protease. The currently practiced alkali cleaning‐in‐place ( CIP ) method was also optimised, and while the optimised protease CIP resulted in complete removal of biofilm cells, the reference CIP (with alkali) resulted in a reduction of 4.08 log biofilm cells/cm 2 and the optimised alkali CIP resulted in a reduction of ∼4.92 log biofilm cells/cm 2 . Furthermore, the amount of the biofilm matrix removed in the optimised protease CIP was significantly higher than that of alkali CIP . It was concluded that the optimised protease CIP has better applicability.