Optimisation of  Bacillus cereus  biofilm removal in the dairy industry using an  in vitro  model of cleaning‐in‐place incorporating serine protease | Zendy

Kumari Sarita | Zendy; Sarkar Prabir K | Zendy

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Optimisation of Bacillus cereus biofilm removal in the dairy industry using an in vitro model of cleaning‐in‐place incorporating serine protease

Author(s) -

Kumari Sarita,

Sarkar Prabir K

Publication year - 2018

Publication title -

international journal of dairy technology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.061

H-Index - 53

eISSN - 1471-0307

pISSN - 1364-727X

DOI - 10.1111/1471-0307.12454

Subject(s) - biofilm , bacillus cereus , protease , serine protease , chemistry , microbiology and biotechnology , cereus , response surface methodology , food science , matrix (chemical analysis) , pulp and paper industry , biology , bacteria , chromatography , biochemistry , enzyme , engineering , genetics

This article addresses a major hygienic issue in the dairy industry, namely biofilm removal. Response surface methodology was deployed to optimise Bacillus cereus biofilm removal conditions using serine protease. The currently practiced alkali cleaning‐in‐place ( CIP ) method was also optimised, and while the optimised protease CIP resulted in complete removal of biofilm cells, the reference CIP (with alkali) resulted in a reduction of 4.08 log biofilm cells/cm 2 and the optimised alkali CIP resulted in a reduction of ∼4.92 log biofilm cells/cm 2 . Furthermore, the amount of the biofilm matrix removed in the optimised protease CIP was significantly higher than that of alkali CIP . It was concluded that the optimised protease CIP has better applicability.

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