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Applicability of protein estimation methods for assaying glycomacropeptide
Author(s) -
Narwal Vikrant,
Sharma Neelima,
Sharma Rajan,
Rajput Yudhishthir S,
Mann Bimlesh
Publication year - 2018
Publication title -
international journal of dairy technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.061
H-Index - 53
eISSN - 1471-0307
pISSN - 1364-727X
DOI - 10.1111/1471-0307.12452
Subject(s) - biuret test , bicinchoninic acid assay , kjeldahl method , lowry protein assay , chromatography , bradford protein assay , chemistry , nutraceutical , absorbance , food science , biochemistry , nitrogen , urea , organic chemistry , casein
Glycomacropeptide ( GMP ) is a nutraceutical additive, which is released during cheese preparation. In this study, the protein content in a laboratory GMP preparation and two commercial GMP samples, which was estimated by the Bradford, biuret, Lowry and bicinchoninic acid ( BCA ) assays, were compared using the Kjeldahl method. There were apparent differences in the values of estimated proteins using the different methods. An absence of dose response in the Bradford method and a low dose response using the Lowry method indicated the unsuitability of both these methods. Absorbance in the biuret and BCA methods linearly increased with increasing GMP content. The BCA method was the most sensitive of these methods.