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Simultaneous detection and identification of pathogenic Cronobacter species by high‐resolution melting analysis in powdered infant formulas
Author(s) -
Hu Shuangfang,
Yu Yigang,
Wu Xinwei,
Xia Xingzhou,
Xiao Xinglong,
Wu Hui
Publication year - 2018
Publication title -
international journal of dairy technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.061
H-Index - 53
eISSN - 1471-0307
pISSN - 1364-727X
DOI - 10.1111/1471-0307.12410
Subject(s) - cronobacter , high resolution melt , infant formula , detection limit , cronobacter sakazakii , microbiology and biotechnology , biology , chromatography , food science , chemistry , gene , polymerase chain reaction , escherichia coli , enterobacter , genetics
A combination method of real‐time PCR and high‐resolution melting ( HRM ) analysis for the rapid detection and specific classification of six pathogenic Cronobacter species based on gene cgcA was developed. HRM profiles with distinct T m (melting temperature) peaks were consistently obtained with each species, represented by a single peak ranging from 86.02 to 86.80 °C. The detection limit ranged from 0.1 to 1 pg per reaction. Desiccated Cronobacter sakazakii and Cronobacter muytjensii of 2 cfu /25 g were successfully detected after 4‐week storage at room temperature. The newly developed method provided a molecular tool for direct detection and simultaneous identification of pathogenic Cronobacter species in powdered infant formulae.