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Confirmation of buffalo tallow in anhydrous cow milk fat using gas liquid chromatography in tandem with species‐specific polymerase chain reaction
Author(s) -
Sharma Vivek,
Hazra Tanmay,
Kandhol Rakesh,
Sharma Rekha,
Arora Sumit
Publication year - 2018
Publication title -
international journal of dairy technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.061
H-Index - 53
eISSN - 1471-0307
pISSN - 1364-727X
DOI - 10.1111/1471-0307.12390
Subject(s) - tallow , anhydrous , cow milk , chemistry , food science , polymerase chain reaction , gas chromatography , chromatography , biochemistry , organic chemistry , gene
Confirmation of buffalo tallow in anhydrous cow milk fat (cow ghee) is a major problem to date. In the present investigation, a novel strategy has been developed to detect and confirm the buffalo tallow in cow ghee. It involved coupling the gas liquid chromatography ( GLC ) of triglycerides with the rapid species‐specific polymerase chain reaction ( PCR ) to confirm the presence of buffalo tallow in anhydrous cow milk fat. Adulteration of cow ghee with buffalo tallow at 10% level could be confirmed using the standardised protocol. The standardised protocol can help in countering the cases of cow ghee adulteration with buffalo tallow.