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Isolation and molecular identification of yeasts in Greek kefir
Author(s) -
Kalamaki Mary S,
Angelidis Apostolos S
Publication year - 2017
Publication title -
international journal of dairy technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.061
H-Index - 53
eISSN - 1471-0307
pISSN - 1364-727X
DOI - 10.1111/1471-0307.12329
Subject(s) - kefir , yarrowia , yeast , debaryomyces hansenii , biology , kluyveromyces , pichia , rhodotorula , food science , trichosporon , microbiology and biotechnology , saccharomyces cerevisiae , bacteria , biochemistry , pichia pastoris , genetics , recombinant dna , lactic acid , gene
The aim of this study was to enumerate and identify yeasts present in Greek kefir. Total yeast counts in kefir grains were 7.7 ± 0.5 log cfu/g, whereas in kefir drinks counts ranged from <0.4 to 6.7 log cfu/ mL . Molecular identification tests revealed 14 different yeast species belonging to 10 genera ( Candida spp., Debaryomyces spp., Galactomyces spp., Issatchenkia spp., Kazachstania spp., Kluyveromyces spp., Pichia spp., Saccharomyces spp., Wickerhamomyces spp., Yarrowia spp.). This study is the first to identify yeast species present in Greek kefirs and to isolate Pichia membranifaciens and Candida zeylanoides as part of the kefir microbiota.