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Rapid lactate oxidase‐based assay for lactate content in milk to ascertain its hygienic quality
Author(s) -
Kumar Brajesh,
Sharma Rajan,
Thakur Rahul,
Barui Amit Kumar,
Rajput Yudhishthir S,
Mann Bimlesh
Publication year - 2016
Publication title -
international journal of dairy technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.061
H-Index - 53
eISSN - 1471-0307
pISSN - 1364-727X
DOI - 10.1111/1471-0307.12285
Subject(s) - chemistry , raw milk , absorbance , peroxidase , benzoic acid , food science , chromatography , lactic acid , glucose oxidase , oxidase test , biochemistry , enzyme , bacteria , biology , genetics
A rapid method for qualitative and quantitative assaying of l ‐lactate in milk is described. The method is based on oxidation of lactate by lactate oxidase with the simultaneous formation of H 2 O 2 which subsequently oxidises 2, 4, 6‐tribromo 3‐hydroxy benzoic acid ( TBHBA ) in the presence of peroxidase. Oxidised TBHBA then reacts with 4‐amino antipyrine to obtain magenta colour (λ max = 523 nm). In the qualitative assay, colour change becomes visible in raw milk samples spiked with 300 mg lactate/L. The absorbance increases linearly up to 1000 mg/L. The method can be used for rapid assessment of the hygienic quality of milk.