Purification of two bacteriocins produced by   E nterococcus faecalis  DBFIQ E24 strain isolated from raw bovine milk | Zendy

Manzo Ricardo M. | Zendy; Cardoso María de las Mercedes | Zendy; Tonarelli Georgina G. | Zendy; Simonetta Arturo C. | Zendy

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Purification of two bacteriocins produced by E nterococcus faecalis DBFIQ E24 strain isolated from raw bovine milk

Author(s) -

Manzo Ricardo M.,

Cardoso María de las Mercedes,

Tonarelli Georgina G.,

Simonetta Arturo C.

Publication year - 2016

Publication title -

international journal of dairy technology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.061

H-Index - 53

eISSN - 1471-0307

pISSN - 1364-727X

DOI - 10.1111/1471-0307.12258

Subject(s) - enterococcus faecalis , bacillus cereus , strain (injury) , molecular mass , bacteriocin , antimicrobial , microbiology and biotechnology , chemistry , food science , raw milk , enterotoxin , escherichia coli , bacteria , biology , biochemistry , gene , enzyme , genetics , anatomy

Enterococcus faecalis DBFIQ E24 was formerly characterised as a producer of antibacterial and antifungal substances. This strain is vancomycin sensitive, nonhaemolytic and gelatinase negative. SDS ‐ PAGE revealed the presence of an active band located between 1060 and 3500 Da. After the application of a three‐step purification procedure, two antimicrobial peptides were isolated. One of them is mostly active against Bacillus cereus with a molecular mass of 1364 Da, and the other one with a molecular mass of 1686 Da is mainly active against Escherichia coli . These results confirm the technological potential of E. faecalis DBFIQ E24 strain as a GRAS food biopreservative.

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