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Purification of two bacteriocins produced by E nterococcus faecalis DBFIQ E24 strain isolated from raw bovine milk
Author(s) -
Manzo Ricardo M.,
Cardoso María de las Mercedes,
Tonarelli Georgina G.,
Simonetta Arturo C.
Publication year - 2016
Publication title -
international journal of dairy technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.061
H-Index - 53
eISSN - 1471-0307
pISSN - 1364-727X
DOI - 10.1111/1471-0307.12258
Subject(s) - enterococcus faecalis , bacillus cereus , strain (injury) , molecular mass , bacteriocin , antimicrobial , microbiology and biotechnology , chemistry , food science , raw milk , enterotoxin , escherichia coli , bacteria , biology , biochemistry , gene , enzyme , genetics , anatomy
Enterococcus faecalis DBFIQ E24 was formerly characterised as a producer of antibacterial and antifungal substances. This strain is vancomycin sensitive, nonhaemolytic and gelatinase negative. SDS ‐ PAGE revealed the presence of an active band located between 1060 and 3500 Da. After the application of a three‐step purification procedure, two antimicrobial peptides were isolated. One of them is mostly active against Bacillus cereus with a molecular mass of 1364 Da, and the other one with a molecular mass of 1686 Da is mainly active against Escherichia coli . These results confirm the technological potential of E. faecalis DBFIQ E24 strain as a GRAS food biopreservative.