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Production of bacteriocin‐like inhibitory substances ( BLIS ) by Bifidobacterium lactis using whey as a substrate
Author(s) -
Balciunas Eduardo Marcos,
Al Arni Saleh,
Converti Attilio,
Leblanc Jean Guy,
Oliveira Ricardo Pinheiro de Souza
Publication year - 2016
Publication title -
international journal of dairy technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.061
H-Index - 53
eISSN - 1471-0307
pISSN - 1364-727X
DOI - 10.1111/1471-0307.12247
Subject(s) - bifidobacterium animalis , fermentation , food science , laboratory flask , yeast , chemistry , yeast extract , bacteriocin , lactose , lactococcus lactis , inulin , bacteria , nisin , microbiology and biotechnology , biology , bifidobacterium , biochemistry , lactobacillus , lactic acid , genetics
The objective of this work was to evaluate the production of bacteriocin‐like inhibitory substances ( BLIS ) by Bifidobacterium animalis subsp. lactis in whey supplemented with yeast extract, inulin, Tween‐80 or l ‐cysteine. Cell growth, acidification, glucose and lactose consumption as well as BLIS production were measured during fermentations carried out in shake flasks. The best additive for both cell growth and BLIS production was shown to be yeast extract, which gave the highest concentrations of biomass (9.9 log cfu/mL) and BLIS (800 AU/mL). In a bench‐scale fermentor, B. lactis growth and BLIS production were between 6% and 25% higher than in flasks depending on the conditions assayed.